International Journal of Research Studies in Agricultural Sciences
Volume-3 Issue-4, 2017, Page No: 1-8
Explant Growth of Forage Palmcv Giant on Different Concentrations of Auxin and Cytokinin
Luciana Cardoso NogueiraLonde1, Marcela Caroline Batista da Mota2, Selma Silva Rocha3, Júlio César Gomes Pereira3, Cintia Maria Teixeira Fialho4, Jéssica Guerra Calaes5
1.Empresa de PesquisaAgropecuária de Minas Gerais, Unidade Regional Epamig Norte de Minas. Rodovia MGT 122, Km 155, Caixa Postal 12, CEP 39527-000 Nova Porteirinha, MG Brazil.
2.Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina - MG.Brazil.
3.Universidade Estadual de Montes Claros, Janaúba – MG. Brazil.
4. Universidade Federal de Viçosa, Viçosa – MG. Brazil.
5. Universidade Federal de Minas Gerais, Montes Claros – MG. Brazil
Citation : Luciana Cardoso N L,et.al.,Explant Growth of Forage Palmcv Giant on Different Concentrations of Auxin and Cytokinin.International Journal of Research Studies in Agricultural Sciences . 2017;3(4):1-8.
Abstract
The forage palm is a good option for forage production in areas with water restriction. This kind of plant has great economic importance in several regions of Brazil; an alternative to increase production would be micropropagation technique, which enables rapid acquisition of a large number of plants with disease free in a short time and with high rates of genetic variability. This practice requires use of growth regulators, such as auxin, used to induce the development of nodes, callus formation and adventitious roots, and cytokinins regulate cell division of the aerial parts of the plant and promote the growth of lateral buds. Therefore, the aim of this study was to evaluate the development of explant of spineless cactus grow crops Giant (Opuntia ficus-indica Mill) established in vitro at different concentrations of naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). The experiment was conducted in a completely randomized design in a factorial scheme 6x6 (ANA and BAP). At 30 and 60 days were evaluated height, diameter, number of shoots and roots. The naphthalene acetic acid provided better results for height, diameter with a hormone dose in the range 1.5 to 2 mg L-1. As for BAP hormone dose was 0.5 mg L-1, with higher doses influence negatively on the formation of roots.