This study was designed to examine in-service training needs of principals in instructional supervision in public secondary schools in Ebonyi State. To accomplish the purpose of the study, four specific objectives and four hypotheses were posed to verify the study. The design for the study was the descriptive study design. Data were collected from a sample of 605 out of the population of 1640 teachers randomly selected by stratified proportionate technique across urban and rural schools, in Abakaliki Education, Ebonyi state. A researcher developed instrument titled "In-Service Training Needs of Principals in Instructional Supervision for teachers in Public Secondary School Questionnaire (ITPSTPSSQ)" was used data for collection. Data were analyzed using mean scores, standard (SD) and t-test statistics. Results indicated that a significant difference existed between urban and rural teachers on principals� in-services training need functions on classroom supervision of instruction, monitoring students� achievement, provision and maintenance of instructional materials, and supporting continuous staff development. The study recommends that the school principals as an instructional leader should give internal supervisions of instructions its rightful place in the school for the purpose of staff growth and development.