The dose-dependent anti-apoptotic and neuroprotective effect of (-)-deprenyl (Selegiline, Jumex) is confirmed. The cytoprotective effect may extend to both neuroectodermal and non neuroectodermal somatic cells. The aim of this study was to demonstrate the cytoprotective and anti-apoptotic effect of deprenyl using a liver tumor cell culture (HepG2) and a tumor cell line originated from human breast tumor (MCF-7) and the antioxidant properties on the HepG2 cell line. The dose-effect relationship of (-)-deprenyl was investigated keeping the cells under optimal culture conditions or in serum-deprived medium. (-)-Deprenyl treatment did not exert toxic or anti-proliferating effect on non-starved HepG2 and MCF-7 cells. However, following serumdeprivation the rate of apoptosis was reduced and cytoprotective effect was detected in low dose (1-100 nM) (-)-deprenyl treated cell cultures, compared to the control. In high dose (100 µM) (-)-deprenyl increased apoptotic ratio and reduced viability of the serum deprived cultures. The antioxidant capacity measured by chemiluminometry of HepG2 cells upon (1 pM-10 µM) (-)-deprenyl treatment significantly increased. In MCF7 cells 1nM and 10µM (-)-deprenyl treatment intensified the estrogen receptor transcriptional activity measured by real time PCR.