The aim of the present study was to evaluate the acute and subacute hepatotoxicity of Ehretialaevis (leaves). Methods: Toxicity study of Ehretialaevis was carried out in Swiss female mice after singleingestion of the extract at different doses (acute toxicity) and daily administration forfifteen days (subacute toxicity). Results: The results showed that the LD50 of the extract was higher than 4000mg/kg and subacute treatment showed no change in weight of liver however, ALT, ALP, AST bilirubin and globulin values increased significantly whereas albumin and protein decreased.Histopathological studies showed aggregation of cells in parenchyma and in blood vessels vicinity at 1000mg/kg. At 2000mg/kg vacuolation was present, sinusoidal spaces absent, acute cellular swellingand mild degree of cell necrosis was noted. At 4000mg/kg cellular swelling was found to be increased and large number of polymorphic cells was present in blood vessels, showing necrosis. Extract showed dose related toxic effects on hepatocytes.Conclusions:On the basis of above findings it is concluded that the n- hexane extract of leaves of Ehretialaevis has hepatotoxic effect in mice. Further, chronic toxicity studies are needed in other species and with different extract