A series of diesters (2a-d) were synthesized from diacetyl resorcinol (1) and studied for their antimicrobial activities. In the first step, resorcinol was utilized to prepare diacetyl resorcinol (1), which was then reacted with substituted aryl acids to furnish the title compounds (2a-d). The structures of the synthesized compounds were established on the basis of data obtained from 1H-NMR, Mass and elemental analysis. The antimicrobial activity (minimum inhibitory concentration; MIC) of the compounds (2a-d) was determined against different bacterial and fungal strains