High cholesterol has been attributed to be a risk factor for cardiovascular diseases. Current measures include dietary changes, exercise and therapeutic agents that lower cholesterol levels. However, pharmacologic agents have significant side-effects and limitations. There has been an increasing interest in probiotics which can lower cholesterol. Lactobacillus has been reported to inhibit cholesterol synthesis and promote excretion of acidic sterol. In this study, Lactobacillus brevis was investigated for its cholesterol lowering effect. To evaluate the cholesterol assimilating property of Lactobacillus brevis, MRS broth with different levels of water-soluble cholesterol was added to culture media and the cholesterol concentration of supernatant broth and pellets were analysed after 24 hours incubation. The results of all MRS broth showed significant reduction of cholesterol both in the supernatant and pellet. This result provides an initial screening of Lactobacillus brevis for their efficacy as cholesterol-lowering therapeutics and demonstrates their potential use as cholesterol-lowering agents.