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DOI Prefix 10.20431 |
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Rabbit Galig Discovery and its Prokaryotic Recombinant Protein Expression
Enhao Fang2, Shanshan Zhang3, XianyuYang1*, Xin Gao1
2.Jixian Honors College, Zhejiang Agricultural and Forestry University, 666 Wusu Street, Hangzhou, Zhejiang 311300, China.
3.School of Forestry and Biotechnology, Zhejiang Agricultural and Forestry University, 666 Wusu Street Hangzhou, Zhejiang 311300, China.
Citation : Enhao Fang, Shanshan Zhang, XianyuYang, Xin Gao, Rabbit Galig Discovery and its Prokaryotic Recombinant Protein Expression International Journal of Research Studies in Biosciences. 2018, 6(5) : 6-11
In the process of cloning and analyzing rabbit (Oryctolagus cuniculus) galectin-3 (gal-3) cDNA, authors found that its open reading frame (ORF) is 729 bp encoding Gal-3 consisting of 242 amino acid residues, which is consistent with previous report. Meanwhile, two galig ORFs embedded in gal-3 were found, whose encoding polypeptides are completely different from that of Gal-3 but show 58% and 57% homology with human Cytogaligin and Mitogaligin, respectively. So far, the function of apoptosis induction has been reported for human Cytogaligin and Mitogaligin by transfection experiments. In consideration of the importance of further investigation on Cytogaligin and Mitogaligin, and the lack of direct detection of their apoptotic activity with protein samples, prokaryotic expression constructs of rabbit pET-28b-cytogaligin and pET-28b-mitogaligin as well as pET-28b-gal-3 were made in current investigation, however only recombinant Gal-3 and Cytogaligin of rabbit were well expressed.