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DOI Prefix 10.20431 |
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Dna Laddering, Rt-Pcr and in Silico Interaction Studies for Aqueous Leaf Extracts of Ipomoea Sepiaria as Anticancer Agents Towards Pc-3 Cell Lines
Sudhakar Meesala1,Satyanarayana Rentala2*,DSVGK Kaladhar3
2.Department of Biotechnology, GITAM University, Visakhapatnam - 530045
3.Dept.of Microbiology and Bioinformatics, Bilaspur University, Bilaspur (CG)-495001
Citation : Sudhakar Meesala, Satyanarayana Rentala, DSVGK Kaladhar, Dna Laddering, Rt-Pcr and in Silico Interaction Studies for Aqueous Leaf Extracts of Ipomoea Sepiaria as Anticancer Agents Towards Pc-3 Cell Lines International Journal of Research Studies in Biosciences 2017,5(9) : 11-15
Apoptosis is a process of programmed cell death (pcd) involved in the cellular process resulting endonuclease cleavage of DNA into fragments and nucleosomal ladders. The effect of the aqueous leaf extract by Ipomoea sepiaria (family: Convolvulaceae) on PC-3 cell lines were tested for the assessment of toxicity levels at the biochemical level for apoptosis. There is no toxic effect on normal cells as there is no DNA fragmentation observed when the gels in 2μM and 10μM extracts treated with PC-3. The RT-PCR MDR1 and TNFa primes yielded product of 246bp based on comparing bands by the primer. The proteins has shown functional relationships with tumor necrosis factor-mediated signaling pathway, cellular response to tumor necrosis factor, death-inducing signaling complex assembly, regulation of response to external stimulus and extrinsic apoptotic signaling pathway. Studies on DNA Ladder and cleavage through RT-PCR and protein interactions shows the presence of relationships and signaling process between MDR1 and TNF alpha.