Change in body weight and exploratory behavior were determined in mice which were randomly divided into two groups of ten mice each, weighing between 16-21g and consumed normal rat fed (0% powdered tobacco) for the control group and 1g of powdered tobacco mixed with 99g of normal rat fed, making 1% of the diet for the test group for 30 days. Food and water intake as well as body weight change were monitored. The light-dark transition box test was used to assess exploratory behavior. The result showed that the line crosses for the tobacco exposed group was significantly higher (P< 0.01) compared to control. Similarly, the light chamber duration was also significantly higher for the tobacco exposed group (P < 0.001) compared to the control. However, the SAP was significantly lower (P < 0.001) compared to control. The frequency of rearing did not differ among the groups. There was an increase in Food intake, water intake and body weight in the test group (P < 0.01) compared to control. Thus, mice exposed to powdered tobacco diet enhance exploratory behavior and increase body weight.