This study evaluated the bioactive compounds in leaves and stem-bark extracts of Piptadeniastrum africanum (Hook.f.) Brenan. Specimens of the plant were obtained from the tropical rain forest in several communities in the eight local government area of Bayelsa State, Nigeria. The samples were extracted using water, and the bioactive constituents were analyzed using qualitative and quantitative techniques. The quantitative results revealed that Alkaloids, Saponins, Flavonoids, Glycosides, Phytate, Tannins, Oxalate and Phenols concentrations were 5.08%, 17.60%, 5.72%, 0.90mg/kg, 0.0917%, 2.684%, 36.55 mg/g and 376.08 mg/kg, respectively in the leaves and 3.00%, 13.60%, 7.34%, 0.50mg/kg, 0.0917%, 2.732%, 61.02 mg/g and 376.08 mg/kg, respectively in the stem-bark. The qualitative results showed that saponins, and terpenes were very high and tannins, steroids, phenol and flavonoids were fairly present in the leaves of P. africanum. While in the stem bark saponins was very high, phenol was high, and tannins, terpenes, steroids and flavonoids were fairly present. The concentrations of the bioactive ingredients varied based on plant parts for some bioactive compounds. Both the qualitative analysis and quantitative determination of the metabolites showed that the plant under study is rich in phytochemicals. Therefore, there is need for further research to focus on other medicinal properties of the plant parts.