Most of the drug industries depend on medicinal plants for the production of pharmaceutical compounds, most herbal medicines and their derivatives products were often prepared from crude extracts from plants; which comprise a complex mixture of different phytochemical constituents (plant secondary metabolites). The aim of the work is to identify the bioactive compounds present in the chloroform extract of Ageratum conyzoides whole plant using Gas Chromatography and Mass spectroscopy. The GC-MS revealed seventy one phytochemicals ranging from high and low molecular weight chemical entities with varying quantities present. The major compounds present include 9,12-Octadecadienoic acid (12.48%), cis-4-Acetoxytrans-1-(m-methoxyphenyl)cyclohexanecarbonitrile (11.52%), Octadecanoic acid (4.97%), Tetratetracontane (1.35%), 1H-Indole-3-carboxaldehyde (3.68%), 5-methoxy-19-Hydroxy-3-alpha,5-cyclo-5alpha-androstan17-one (1.44%), cis-4,4-Dimethylbicyclo(6.3.0) undecane-2,6-dione (1.89%), 2-Heptenoic acid (1.23%), Kaur-16-ene (1.04%), Bicyclo [11.3.0] hexadecane-2,14-dione (0.45%), 3,3,6,6-Tetramethyl-1,2,3,4,5,6,7,8- octahydroacridine (1.61%). The minor ones such as Benzenemethanol, 4 -(1,1-dimethylethyl)- (0.28%), Benzaldehyde, 2-hydroxy-4-methoxy-(0.22%), 2,3,4,7-Tetrahydro-1H-indene (0.18%), 7-Oxabicyclo [4.1.0] heptanes (0.16%), 2-Undecanone, 6,10-dimethyl- (0.96%), Diazoacetic acid, 2-isopropyl-5-methylcyclohexyl ester (0.54%), Bicyclo[11.3.0]hexadecane-2,14-dione (0.45%), are also present. These chemical compounds are considered biologically and pharmacologically important.