Present study conducted the preliminary run extracting the bio-oil in Padina sp. using conventional technique. The processing of bio-oil was conducted in Cebu Technological University - Carmen campus on August 9 to 15, 2017. Results showed that the weights in gram of bio-oil obtained per replicates; 9.93g for 25g, 21.30g for 50g, and 32.30 for 75g.Overall percent yield was 41.80% for the total biomass, 450g.Further studies needed to verify the bio-oil in the samples for future bio-diesel production.