The side effects and the increasing microbial resistance to synthetic drugs in the management and treatment of infections remain an issue in modern medicine, hence the increasing research in phytomedicine. Therefore in the present study, the active phytocomponents of Mimosa pudica's wholesome parts were revealed using quantitative phytochemical analysis. The antibacterial activity of the Ethanolic extract was studied using Well diffusion method. The activity was tested against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeroginosa, Escherichia coli and Mycobacterium smegmatis at different concentrations of 25, 50 and 100 mg/disc and the results have been illustrated.