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DOI Prefix 10.20431 |
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International Journal of Medicinal Plants and Natural Products
Volume-1 Issue-2, 2015, Page No:35-43
The Anthelmintic and Antioxidant Activities of South African Geranium Incanum
Babajide Jelili Olalekan1*, Green Ivan Robert3, Mabusela Wilfred Thozamile2
1.Department of Chemistry, University of the Western Cape, Private Bag X17, Bellville 7535, Cape Town, South Africa.
2.South African Herbal Science and Medicine Institute, University of the Western Cape,Private Bag X17,Bellville 7535, Cape Town, South Africa.
3.Department of Chemistry, University of Stellenbosch, Private Bag X1, Matieland, Stellenbosch 7600, South Africa.
2.South African Herbal Science and Medicine Institute, University of the Western Cape,Private Bag X17,Bellville 7535, Cape Town, South Africa.
3.Department of Chemistry, University of Stellenbosch, Private Bag X1, Matieland, Stellenbosch 7600, South Africa.
Citation : Babajide Jelili Olalekan,Green Ivan Robert,Mabusela Wilfred Thozamile, The Anthelmintic and Antioxidant Activities of South African Geranium Incanum International Journal of Medicinal Plants and Natural Products. 2015;1(2):35-43.
Abstract
In screening several plant species from an inventory of common medicinal plants from South Africa for diverse medicinal purposes, Geranium incanum plant was selected for investigation because of its interesting ethnomedicinal profile. The present study was designed to evaluate the anthelmintic and antioxidant potentials in the different extracts and Isolates of the South African G. incanum. The anthelmintic activity was carried out using larval stage paralysis method and levamisole as reference drug. The larvae stage of Haemonchus contortus from faecal samples of infected rabbits that was obtained from cultured specimen was used for the analysis. The different extracts showed different level of inducement with the methanolic extract, induced approximately 85% larval paralysis within 24 h of contact with the H. contortus larvae at 20 mg/ml. This level of activity may be useful in animal husbandry practice to encourage animals to develop immunity against subsequent worm infections. This action gives a good ground for the use of the plant for treatment of helminthiasis in African traditional medicine. The total antioxidant assay also reveal a huge amount of excellent information into the nature and properties of each extracts and isolates in which the distribution of polyphenols, flavonoids and anthocyanins present in the plant extracts were revealed. The ORAC, FRAP and TEAC showed intensively the antioxidant capacity of the extracts and isolates in which it was evident that the methanolic extracts and isolates obtained from them are good antioxidant agents for which G10 is even higher than the standard used in some case as shown in the results obtained. The total phenol varied from 14.1±1 to 298.5±5 mg/g in the extracts. Flavonoid contents were between 22.15±0.8 and 81.3±5.4 mg/g. The methanolic extract has higher ORAC and phenolic contents as compared to other extracts. The highest radical scavenging effect was observed in the methanolic extract with IC50 = 0.014 mg/ml. The potency of radical scavenging effect of the methanolic extract was about 5 times greater than a synthetic antioxidant butylated hydroxy toluene (BHT). Due to these 2 observed properties, the extracts of the plant might be used as a new multifaceted drug. The data obtained is a promising profile for development of a good anthelmintic and antioxidant agents for the future, especially compounds G3 and G10 as a single dose for both anthelmintic and antioxidant therapy.
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