Delayed fertilization can be a tool to improve salmonid spawning efficiencies. This study subjected eggs from landlocked fall Chinook salmon (Oncorhynchus tshawytscha) to one of three treatments: fertilization immediately after egg removal from female brood stock, fertilization delayed for two hours postegg removal, or fertilization delayed for four hours. There were no significant differences in egg survival to the eyed-stage or fry hatch between the immediate fertilization and two-hour delay treatments. However, survival to the eyed-stage and survival to fry hatch were significantly reduced in the eggs fertilized after a four-hour delay. These results indicate that landlocked fall Chinook Salmon eggs can be successfully stored for up to two hours prior to fertilization with no negative effects on egg survival.