We investigated the effects of a preparation by wet nanotechnology on the pollen, pistil, and fruit loss in ginkgoes. Pollen germination in the test medium was investigated by adding insoluble calcium and magnesium nano particles. The germination rate increased 30% after 72 h; however, pollen in germination medium to which 1 μL agitating agent was added did not germinate after 72 h. Thus, the nanoized emulsifier inhibited germination of ginkgo pollen. Fruit loss rate for ginkgo seedlings sprayed with nanoized reding agent decreased by 60-90% more than that of unsprayed fruit. However, 4 days following the application of the re-deposition agent, the content of calcium and magnesium in the ginkgo pistils decreased, requiring reapplication within 3 days. The soil pollution test confirmed the enrichment agent did not affect surrounding soil, and it will be applicable to various roadside trees in addition to ginkgoes. Because the external environment may affect success (e.g., spraying method, rain, and wind),it is necessary to set a spraying and reapplication time. The wet nano-process technology is expected to be applicable to various fields,and can be used in natural materials and bioindustries, as well as for plant nutrients.