Submit Paper

Article Processing Fee

Pay Online

           

Crossref logo

  DOI Prefix   10.20431


 

International Journal of Forestry and Horticulture
Volume 4, Issue 2, 2018, Page No: 1-6
doi:http://dx.doi.org/10.20431/2454-9487.0402001

Method Optimization Studies on Inoculation and Isolation of the Causal Agent of Lettuce Downy Mildew (Bremia Lactucae)

Razieh Ebrahimzadeh1,F.Sara Dolar19,Kenan Sonmez2,S.Sebnem Ellialtioglu3

1.Ankara University, Faculty of Agriculture, Department of Plant Protection, Ankara, Turkey.
2.Eskisehir Osmangazi University, Faculty of Agriculture, Department of Horticulture, Eskisehir, Turkey.
3.Ankara University, Faculty of Agriculture, Department of Horticulture, Ankara, Turkey.

Citation : Razieh Ebrahimzadeh,et.al, Method Optimization Studies on Inoculation and Isolation of the Causal Agent of Lettuce Downy Mildew (Bremia Lactucae) International Journal of Forestry and Horticulture 2018, 4(2) : 1-6

Abstract

Downy mildew disease caused by Bremia lactucae causes one of the most devastating diseases of lettuce worldwide. An obligat parasite, B.lactucae's sporulation in unnatural environments and storage is more difficult than necrotrophic pathogens and some special methods are needed to be developed. For this aim, disease samples were collected during 2016 lettuce growing seasons. The all samples were stored at -20 °C and -80 °C. Small leaf parts showing symptoms from the samples were dissected and spores were scraped by various methods. The spore suspensions were obtained using three methods (keep at room illumination+room temperature, room illumination+stored in ice, darkness+room temperature). In order to determine the suitable inoculation method, seedling, and detached leaf tests (blotter, floating, waxed slide test and water agar methods) were performed. A series of petri plates and seedlings were kept at 13±1 °C in the climate chamber and another series of assay were incubated at 18-21 °C until sporulation.

Among the inoculation methods used in the present study, the best results were obtained from waxed slide and MS+Agar methods. Both incubation temperatures were found to be appropriate and there was no significant difference between them.


Download Full paper: Click Here