Submit Paper

Article Processing Fee

Pay Online

           

Crossref logo

  DOI Prefix   10.20431


 

International Journal of Forestry and Horticulture
Volume 3, Issue 3, 2017, Page No: 28-36
doi:http://dx.doi.org/10.20431/2454-9487.0303005

Studies for Obtaining the Protocorms and Plantlets in Orchis pinetorum, Anacamptis pyramidalis, and Dactylorhiza nieschalkiorum under in vitro Conditions

Cevdet GUMUS1,Sekure Sebnem ELLIALTIOGLU2,Senel Birceyudum EMAN3

1.Bartin University, Vocational School, Bartin, Turkey
2.Ankara University, Faculty of Agriculture, Deparment of Horticulture, Ankara, Turkey

Citation :Cevdet GUMUS,Sekure Sebnem ELLIALTIOGLU,Senel Birceyudum EMAN, Studies for Obtaining the Protocorms and Plantlets in Orchis pinetorum, Anacamptis pyramidalis, and Dactylorhiza nieschalkiorum under in vitro Conditions International Journal of Forestry and Horticulture 2017,3(3) : 28-36

Abstract

Orchis pinetorum, Anacamptis pyramidalis, and Dactylorhiza nieschalkiorum protocorms and plantlets were obtained via asymbiotic germination under in vitro conditions. For the germination of seeds, 4 basic nutrient media consisting of half-strength MS (1/2MS), full-strength MS, Van Waes DeBergh (VW and DB), and Knudson C. The petri dishes contained orchid seeds and the nutrient medium semi-solidified with agar (0.6%). cultures were incubated in darkness for the first 3 months and thereafter transferred to a light/dark photoperiod of 16/8 hours. At the end of the third month, protocorm rates were identified. They are subcultured every 4 weeks and 1/2 MS medium was used as a transfer medium. The highest germination rate,protocorm rate and plant growth rate were obtained from Knudson C medium.


Download Full paper: Click Here