In this study, the phytoalexin accumulation in plant tissues was investigated using the callus culture technique in eggplants with the treatments a Verticillium dahliae elicitor. For this purpose, one susceptible cultivar Solanum melongena L. cv. Long Purple and one resistant wild species S. sisymbriifolium to Verticillium wilt were used as the plant materials. Elicitors were applied to the calluses obtained from the hypocotyl explants of both the species. Autoclaved spore suspensions of V. dahliaewere used as the elicitor. The elicitation was stopped at 24, 48, and 72 h after the elicitor application. The callus extracts were analyzed by gas-liquid chromatography. The V. dahliaeelicitor was induced by a solavetivone phytoalexin accumulation in the eggplant callus tissues under in vitro conditions. The highest accumulations were obtained from a '2.0 mL V. dahliae* 72 h after elicitation' treatment and the 'dose * time after elicitation' interactions were found to be statistically significant. At the highest V. dahliae elicitor application dosage, the amount of solavetivone in the callus cultures of the resistant species was higher than the amount in the susceptible cultivar. The phytoalexins could be very effective in the resistance metabolism of eggplants against to V. dahliae.