This work was undertaken to compare yoghurt produced from unpasteurized milk, laser pasteurized milk and heat pasteurized milk. Milk samples were obtained from SUST farms, Shambat, North Khartoum, Sudan. ND: YAG laser used to pasteurize the first sample, the second sample pasteurized by heat and the third one was left as obtained to control sample. Yoghurt is produced by bacterial fermentation of milk. The results showed that the use of laser in pasteurization is not differed from heat pasteurization, while the production of yoghurt from laser pasteurized milk is slightly better than that of heat pasteurized milk.