The physico-chemical parameters of water samples collected from river mandakini at various locations of chitrakoot District were assess. Physico-chemical parameters like, Temperature, pH, Alkalinity, TDS, Chloride, Total Hardness, Sulphate and Phosphate was determined. The river mandakini originates near village kalhura in the majhgawan block, district Satna of Madhya Pradesh at latitude 24°52'N and longitude 80° 41'E.The results were compared with standards prescribed by WHO (1984). Temperature, pH, Chloride, Sulphate and phosphates of all the samples were found below the permissible limit set by WHO. It is concluded that the water of river is not highly polluted but there is an indicating of increasing pollutant due to anthropogenic activities. Proper monitoring is needed to avoid anthropogenic contamination.