The leaf of Palicourea croceiodes (Rubiaceae) was extracted with ethyl acetate using cold maceration method, and the extract was subjected to preliminary phytochemical screening using standard methods and antimicrobial assay against some pathogenic bacteria and fungi including Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Klebsiella aerogenes, E.coli, Proteus mirabilis, Enterobacter aerogenes, Candida albican and Aspergillusniger. Using the agar well diffusion method. The phytochemical screening revealed the presence of terpenes, acidic compounds, saponins, flavonoids and glycosides. The extract was also found to exhibit selective activities against some of the organisms with Minimum Inhibitory Concentration (MIC) of between 0.25mg/ml and 0.0625mg and a Minimum Fungicidal Concentration of (MFC) of 0.125mg/ml and 0.0625mg/ml. The study justified the use of the plant as an antibiotic in ethno-medical applications.