Abstract

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A ~1.9kb cDNA containing the entire coding region, 5’ UTR, and 3’ UTR is cloned from rat tumor hepatoma (KRC-7) cells. It was further characterized for protein expression in rabbit reticulocyte lysates. Later, this cDNA was used for extensive mutagenesis and biochemical studies to identify specific domains/sites of rat p67 that are essential for its functions. To investigate whether similar size transcript is also present in mouse tissues, we examined the tissue distributions of mouse p67 transcript and protein from male and female mice. We found that there are two mouse p67 transcripts – one is ~1.4kb long whereas the other is ~2.2 kb long. The ~1.4 kb long transcript is present in almost all tissues tested whereas the ~2.2 kb transcript is present in brain, spleen, skeletal muscle, and possibly in lungs. Our analysis for the tissue distributions of p67 protein detected several proteins corresponding to ~67 kDa, ~60 kDa, 52 kDa, 26 kDa, and possibly others in different proportions in various tissues of both male and female mice.

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