The enzyme activities super oxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (As-POX), peroxidase (POX) and glutathione reductase (GR) and phenolic compounds were studied in tuber and shoot tissues from Ceropegia spiralis, C. pusilla and C. juncea during acclimatization under three different environmental conditions: open field, (35°C-40°C), green house (25°C-30°C) and in culture room (20±2°C). Significant increase in SOD activity in tubers was discernible with increase in temperature. It is interesting to note that, CAT and GR activities in shoots and tubers differed contrastingly in their patterns with reference to temperature increase wherein a significant reduction in CAT and increase in GR activity were associated with increased tempereture. The variation was more prominent in the shoot tissues rather than in tubers. However, there was not much significant difference among the species with regard to treatments in tubers. Similarly As POX activity, POX activity and phenolics were significantly lower at in vitro conditions both in shoots and tubers and in green house conditions the same were found to be increased.