Lipase is an enzyme that catalyzes the hydrolysis of ester bonds in substrates such as phospholipids, triglycerides and cholesteryl esters. Lipase (E.C. 3.1.1.3) was extracted from the 5 th day of germination of bambara groundnut (Voandzeia subterrenea) using 0.1M phosphate buffer pH 7.8. The enzyme was purified using 60% ammonium sulphate saturation via precipitation followed by dialysis and column chromatography using carboxymethyl cellulose. Lipase from germinated voandzeia subterrenea has various industrial applications hence alternative source of the enzyme is crucial especially from plant.