Prevalence of Vibrio parahaemolyticus in Penaeus Monodon
(Fabricius, 1798) from the Douala Coastal Waters of Cameroon:
Implication for Food Safety

Rhoda Nsen Bughe; Pius Mbu Oben; Benedicta O. Oben; Akindeh Mbuh Nji; Innocent Mbulli Ali; Palmer Masumbe Netongo; Wilfred Fon Mbacham

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International Journal of Research Studies in Biosciences

Volume 4, Issue 6, 2016, Page No: 10-20
doi.org/10.20431/2349-0365.0406003

Prevalence of Vibrio parahaemolyticus in Penaeus Monodon (Fabricius, 1798) from the Douala Coastal Waters of Cameroon: Implication for Food Safety

Rhoda Nsen Bughe¹ ⁴ ⁵, Pius Mbu Oben², Benedicta O. Oben¹, Akindeh Mbuh Nji¹ ⁶, Innocent Mbulli Ali⁶ ⁷, Palmer Masumbe Netongo² ⁶, Wilfred Fon Mbacham* ³ ⁵ ⁶

1.Fisheries programme, Department of Animal Science, Faculty of Agriculture and Veterinary Medicine, University of Buea, Cameroon.
2.Department of Biochemistry, Faculty of Science, University of Yaounde 1, Cameroon.
3.Department of Biochemistry and Physiology, Faculty of Medicine and Biomedical Science, University of Yaounde 1, Cameroon.
4.Department of Biological Sciences, Higher Training College, University of Yaounde 1, Cameroon.
5.Food and Drug Safety Unit (FODRUS), Biotechnology Center, Nkolbisson University of Yaounde 1, Cameroon.
6.The Laboratory for Public Health Research Biotechnologies, Biotechnology Center, Nkolbisson University of Yaounde 1. Cameroon.
7.Department of Biochemistry. Faculty of Science, University of Dschang, Cameroon.

Citation :Rhoda Nsen Bughe , Pius Mbu Oben, et. al, Prevalence of Vibrio parahaemolyticus in Penaeus Monodon (Fabricius, 1798) from the Douala Coastal Waters of Cameroon: Implication for Food Safety International Journal of Research Studies in Biosciences 2016,4(6) : 10-20.

Abstract

Seafood constitutes an important food component and a cheap source of protein in developing countries. Shrimps are prone to bacterial contamination in their environment and have been frequently implicated in Vibrio parahaemolyticus infection.

Of the 121 Penaeus monodon collected from coastal beaches in Douala, 79(65.3%) were contaminated with Vibrio spp. Presumed Vibrio sp. were identified by standard biochemical tests and further confirmed by API 20E kit.

A total of 150 Vibrio parahaemolyticus were isolated from Penaeus monodon and predominated in the gills40%, 22.7% from hepatopancrea and 37.3% from intestines. V. parahaemolyticus was high in Shrimps from Essengue beach (56%). V. parahaemolyticus occurrence was high in the dry season in Youpwe beach (32.6%) while low in Essengue beach (22%). Meanwhile in the wet season Essengue beach recorded high prevalence (32.7%) of V. parahaemolyticus as opposed to 20.7% in Youpwe beach. A high prevalence of haemolytic activity (80.4%) was observed when V. parahaemolyticus was grown on 5% human blood agar. Complete haemolysis predominated with a prevalence of 47.8% while partial haemolysis (32.6%) and non- haemolysis (19.6%). This study has shown that the Vibrio parahaemolyticus isolated from Penaeus monodon of the Douala coastal waters is pathogenic and can pose a health risk when consumed without proper cooking.

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