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DOI Prefix 10.20431 |
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The Impact of HIV and Plasmodium falciparum Genetic Diversity on Anaemia in HIV-patients Attending Care and Treatment Centre at Baptist Hospital Mutengene
Emmaculate Lum2,Elias Fomunyam Onyoh3,Godlove Bunda Wempnje1,Bate Agbor Ayukenchengamba1,Conica Mfetngwie Njabi1,Pascal Obong Bessong6
2.Department of Biological Sciences, Higher Teachers' Training College, University of Yaounde 1, P.O. BOX 47, Central Region, Cameroon.
3.AIDS Care and Prevention Program, Cameroon Baptist Convention Health Services, Cameroon.
4.Institute of Epidemiology and Preventive Medicine, College of Public Health, National Taiwan University, Taiwan.
5.HIV/AIDS and Global Health Research Programme, Department of Microbiology, University of Venda, Thohoyandou 0950, South Africa
Citation :Emmaculate Lum,et.al, The Impact of HIV and Plasmodium falciparum Genetic Diversity on Anaemia in HIV-patients Attending Care and Treatment Centre at Baptist Hospital Mutengene International Journal of Research Studies in Biosciences 2016,4(5) : 26-37
The aim of this study was to assess the impact of Human Immunodeficiency Virus (HIV) and Plasmodium falciparum genetic diversity on anaemia in HIV-patients attending Care and Treatment Centre in Baptist Hospital Mutengene (BHM). This was a cross-sectional study carried out in BHM from June 2012 to August 2013, consisting of 758 patients. Patients at BHM were interviewed using a structured questionnaire to obtain information on socio-demography. About 3mls of venous blood were collected, thick and thin blood films for parasite detection and speciation respectively were prepared. Hb concentration and WBC counts were measured to determine anaemic and leucopenic status. First and nested PCR were done on Plasmodium falciparum positive samples to determine Merozoite Surface Protein-1 (MSP-1) and MSP-2 allelic variants. More HIV-patients were malaria positive (34.77%), anaemic (31.96%; P< 0.001) and leucopenic (21.13%; P=0.004) as compared to those in malaria mono-infection i.e (16.5%), (3.03%) and (0.00%) respectively. The MSP-1 allelic variants detected were KI (65.22%), MAD20 (27.54%) and RO33 (27.54%) while the MSP-2 allelic variants were FC27 (30.43%) and 3D7/IC (31.88%). HIV could predispose patients to more malaria and anaemia while MOI revealed that genetic diversity can cause anaemia. This can assist care givers for the better management of HIV-patients