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DOI Prefix 10.20431 |
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Extracellular Formation of Silver Nanoparticles by the Cell Filtrate of Actinomyces spp. NSX-333
Kh. G. Ganbarov1, N. A. Agayeva2, M. A. Ramazanov3, S. M. Abdulhamidova1, Z. A. Agamaliyev3, G. M. Eyvazova3
2. Department of Microbiology and Immunology, Azerbaijcan Medical University, Baku, Azerbaijan.
3. Department of Physical Chemistry of Nanomaterials, Baku State University, Baku, Azerbaijan.
Citation : Kh. G. Ganbarov, N. A. Agayeva, et.al, Extracellular Formation of Silver Nanoparticles by the Cell Filtrate of Actinomyces spp. NSX-333 International Journal of Research Studies in Biosciences 2016,4(11) : 57-60.
In this paper, we have used a biological method of the biosynthesis of silver nanoparticles,using supernatant (culture liquid)of bacterium Actinomyces spp.NSX-333,,isolated from Azerbaijani soil.The culture liquid has been obtained by culturing the bacterium at 28 ° C for 5-6 days. It was showed that during the incubation of the culture liquid with AgNO3 solution, a restoration of silver ions takes place and stable silver nanoparticlesare formed. Initially, the nanoparticle formation process was visually observed by a color change of the incubation solution.The nanoparticles have characterized using a UV spectrophotometer, anenergy dispersive X-ray spectroscopy (EDXS) and a scanning electron microscope (SEM).UV spectrophotometric analysis of nanoparticlesshowed the presence of absorption in the range of 410 nm, which is characteristic of silver nanoparticles. EDXS spectrum showed a peak corresponding to the silver nanoparticles. Obtained silver nanoparticles had a spherical shape and size in the range of 20 to 130 nm. Clusters of nanoparticles were also observed.