Comparison of Ferulic Acid and Sildenafil, Therapeutic Role against Testicular Injury Induced by Cadmium Chloride in Adult Male Albino Rat.(A study using Light & Atomic-Force Microscopes "L.M & AFM" and Gene expression by Real-Time PCR)
Azza H.Ali1, Entesar A.Saber2, Rasha F.Ahmad3, Arafat H.F.H.4,El-Demerdash R.S.5
Citation :Azza H. Ali,et.al, Comparison of Ferulic Acid and Sildenafil, Therapeutic Role against Testicular Injury Induced by Cadmium Chloride in Adult Male Albino Rat.(A study using Light & Atomic-Force Microscopes "L.M & AFM" and Gene expression by Real-Time PCR) International Journal of Research Studies in Biosciences 2017,5(1) : 6-15
Cadmium chloride considered one of the toxic heavy metals for both animals and human and can induce damage in animal organsas testis, kidney and liver. Testis is the main organ to be affected by lowering fertility. This was clarified in this study by histological, biochemical and laboratory findings (semen and hormonal analysis). Ferulic acid (FA) is a common polyphenolic compound present in vegetables, as, egg-plants and is an antioxidant preventing lipid oxidation in food and consequently prevents free-radical-induced diseases. Sildenafil citrate (causes dilation of peripheral veins and arteries soused for the treatment of erectile dysfunction).
Results showed that cadmium chloride was found to induce increased testicular tissue roughness using the "AFM" and induced sever damage of seminiferous tubules that might be up to necrosis as seen by using the" L.M". This was associated with increased gene expression of B-Cell/ Lymphoma 2 (Bcl 2) , cyclooxygenase-2 (COX-2) and Metallothionein 1(Mt1) , a significant decrease in inhibin- B level which was associated with insignificant increase in testosterone and significant increase in T3 & T4 levels ,while decreasing count and viability of sperms in semen analysis. Whereas, treatment with ferulic acid as well as sildenafil significantly increased the level of inhibin- B.Treatment with only sildenafil significantly increase the count and viability of sperms, while treatment with ferulic acid only significantly increased the viability of sperms but decreased their count.