Lymnaea stagnalis a pest of aquatic vegetation, prolific breeders and the intermediate host of helminthes diseases. So it is essential to control the fertility. The objective of these study larval stages of Lymnaea stagnalis was treated with different concentration of paclitaxol and colchicine. The values of LC100, LC50, LC0 and sub lethal concentration were detected out and data was summarised in the table. Mortality during larval stages was high in treated group in snail which was more pronounced in trochophore larval stage. Detection of negatively charged protein fraction in the larval stage of control and treated snails by SDSPAGE of Lymnaea stagnalis was assessed in the present investigation.