Gynaeacological disorders are various diseased conditions negatively affecting female reproductive organs. This study was conducted to establish a regional profile of the indigenous knowledge on the treatment of various gynaeacological disorders in Lagos State, Nigeria. Oral and semi-structured interviews were used to obtain information from 100 local informants in five local government areas of Lagos State, Nigeria. Qualitative phytochemical screenings of the medicinal plants were done using standard laboratory procedures. Fifty (50) plant species belonging to 35 families were identified for the treatment of different gynaeacological disorders in the study area. Ethnobotanical uses of 16 plant species for amenorrhea, 9 species for aphrodisiac, 7 species for vaginal infections and 6 plant species for sexually transmitted diseases were discovered in the study area. Leaves were the most commonly used plant part (29.03 %) followed by the bark (22.58 %) while the least commonly used plant part was the bulb (1.61 %). Decoction (48.08 %) was commonly used mode of preparations, followed by powdered form (23.08 %) while taking the plant raw (1.29 %) was the least commonly used method. Phenols and flavonoids were present in all the plants while steroids were present in all the plants except six plants and phlobatannins were present only in ten (10) plant species. This study showed that Lagos State is rich in plant species that contain significant bioactive compounds useful in the treatment of various gynaeacological disorders in Lagos State, Nigeria.